COOMASIE BRILLANT BLUE G 250 manufacturers, suppliers, exporters in Mumbai, Gandhidham, Kolkata, Varanasi, Visakhapatnam, Chennai, Fujairah, Dubai, Canada BC, Barka, Sohar, Muscat, Oman.
Lab chemicals manufacturers, suppliers, exporters in India, UAE Middle East, Barka, Sohar, Muscat, Oman, Canada.
COOMASIE BRILLANT BLUE G 250 is available in small packing as well as in bulk. Buy premium quality COOMASIE BRILLANT BLUE G 250 and other lab chemicals from one of the most trusted brands.
Coomassie Brilliant Blue G-250 (CBBG) is a member of the Coomassie family of,
triphenylmethane dyes and is used as a common analytical dye in SDS-PAGE and BN-PAGE.
CBBG can also be used with the Bradford Method to determine protein concentration.
Brilliant Blue G is a useful dye for SDS gels as it readily stains proteins with minimal background color. Protein bands can be visualized during staining. It has been used in the Bradford dye-binding protein assay. A mechanism for dye binding to protein has been proposed, based onmeasuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant Blue G has been used for determining critical micelle concentration of detergents. Recent research reveals CBBG as a P2X7 (P2X purinoceptor 7: P2RX7) antagonist and suggests that it exhibits neuroprotective properties in rat spinal cord cells.
Coomassie Brilliant Blue R-250 was first used to visualise proteins in 1964 by Fazekas de St. Groth and colleagues. Protein samples were separated electrophoretically on a cellulose acetate sheet. The sheet was then soaked in sulfosalicylic acid to fix the protein bands and then transferred to a solution of the dye.