Malachite green has classically been used by the light microscopist for the demonstration of fungi and bacterial spores. It is a weakly basic diaminotri- phenylmethane dye with useful indicator properties at both very high and very low pH Malachite Green is used for bacterial spore staining by Schaeffer and Fulton`s method. It can also be used in place of methyl green in the Pappenheim stain, when combined with the Gram stain.
Malachite green is used for routine staining methods in bacteriology, e. g. the spore-staining method acc. to Rakette (differential spore staining with malachite green in bacteriological samples). The dye is used in a hot staining method to stain specifically target struc tures. Counterstain with a red dye.
It is used as a biological stain for microscopic analysis of cell biology and tissue samples. In the Gimenez staining method, basic fuchsin stains bacteria red or magenta, and malachite green is used as a blue-green counterstain. ... Malachite green is in a class of molecules called fluorophores.
Typically cures ich in 24 hours, treatment can be repeated in 24-48 hours, if necessary. If fish experience stress, change 50% of the water. Sometimes treatment will take 10 days to fully irradiate infestations.
