MALACHITE GREEN FOR MICROSCOPY, Diamino-Triphenylmethane
Malachite green has classically been used by the light microscopist for the demonstration of fungi and bacterial spores. It is a weakly basic diaminotri- phenylmethane dye with useful indicator properties at both very high and very low pH Malachite Green is used for bacterial spore staining by Schaeffer and Fulton`s method. It can also be used in place of methyl green in the Pappenheim stain, when combined with the Gram stain.
MALACHITE GREEN helps for TREATMENT of DISEASES in FISH
Saprolegnia parasitica is a rapidly spreading and fatal fungal parasite affecting both fish and fish eggs. It is conventionally treated with MALACHITE GREEN (diamino-triphenylmethane), but though this treatment is highly effective, the use of malachite green carries with it a number of potential problems: the compound has been suggested as a possible carcinogen and teratogen, though these effects are as yet unproven; being a strong dye, it tends to discolour water, and can under certain conditions cause staining of fish which have been treated; it has a relatively long withholding period, so that significant residues can be present in treated fish when they are harvested and sold for consumption.
BRONOPOL RXSOL-43-4302-025 also used in Fish medicine: Used in fish medicine.
Malachite green is used for routine staining methods in bacteriology, e. g. the spore-staining method acc. to Rakette (differential spore staining with malachite green in bacteriological samples). The dye is used in a hot staining method to stain specifically target struc tures. Counterstain with a red dye.
It is used as a biological stain for microscopic analysis of cell biology and tissue samples. In the Gimenez staining method, basic fuchsin stains bacteria red or magenta, and malachite green is used as a blue-green counterstain. ... Malachite green is in a class of molecules called fluorophores.
Typically cures ich in 24 hours, treatment can be repeated in 24-48 hours, if necessary. If fish experience stress, change 50% of the water. Sometimes treatment will take 10 days to fully irradiate infestations.
