CAPS buffer is a zwitterionic buffer widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF or nitrocellulose membranes. The high pH of this buffer makes it useful for the transfer of proteins with
The developed fluidic system operates on pneumatic actuations. The closed system ensures a very low probability for contamination during sample processing, which is crucial when using a highly sensitive detection method such as PCR. The enrichment of the bacterial cells is achieved using different types of amine-functionalized particles.
A simple, sensitive, and robust method has been developed for the separation and quantification of glutamate in beverages and foodstuffs. Samples were derivatized with 5 × 10−4 M fluorescein isothiocyanate. Glutamate was separated by capillary electrophoresis and detected by laser-induced fluorescence with 488-nm radiation from an argon ion laser.