Harris Hematoxylin Solutions are nuclear stains intended for use in Histology and Cytology. Harris Hematoxylin Solutions are for “In Vitro Diagnostic Use”. Hematoxylin, a common nuclear stain, is isolated from an extract of logwood (Haematoxylon campechianum).
The Harris Haematoxylin Stain (Ortho) is a specially formulated nuclear stain designed to stain nuclei of cytological preparations. The stained cell sample is used to facilitate the diagnosis of the pathological state intended for in-vitro examination of cellular deformity
Add 50 ml of 10% alcoholic hematoxylin solution and heat to boil for 1 minute. Remove from heat and slowly add 2.5 g of mercuric oxide (red). Heat to the solution and until it becomes dark purple color. Cool the solution in cold water bath and add 20 ml of glacial acetic acid (concentrated).