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HEPES BUFFER A biological buffer (exist in a zwitterionic form ). It maintains physiological pH in cell culture when compared to bicarbonate buffers despite the changes in carbon dioxide concentration.HEPES is a zwitterionic organic chemical buffering agent; one of the twenty Good's buffers.
HEPES BUFFER A biological buffer (exist in a zwitterionic form ). It maintains physiological pH in cell culture when compared to bicarbonate buffers despite the changes in carbon dioxide concentration.HEPES is a zwitterionic organic chemical buffering agent; one of the twenty Good's buffers.
HEPES BUFFER A biological buffer (exist in a zwitterionic form ). It maintains physiological pH in cell culture when compared to bicarbonate buffers despite the changes in carbon dioxide concentration.HEPES is a zwitterionic organic chemical buffering agent; one of the twenty Good's buffers.
HEPES BUFFER A biological buffer (exist in a zwitterionic form ). It maintains physiological pH in cell culture when compared to bicarbonate buffers despite the changes in carbon dioxide concentration.HEPES is a zwitterionic organic chemical buffering agent; one of the twenty Good's buffers.
Prepared from HEPES free acid and cell culture grade water
HEPES has been used as a component of:
- Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices
- Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.
The recommended dosage is 70 mg/m2 irinotecan (4.3 mg/ml) resulting in an average male (1.9 m2) receiving a total amount of 133 mg of the drug and 126 mg of HEPES. This amount of administered HEPES is much higher than the recommended 200 μg per dose for gallium-68 based radiopharmaceuticals
