Lactophenol cotton blue is used in medical mycology for the examination of fungal cell structure, by preparing a wet mount. ... When preparing the microscopic slide, methyl blue stains the chitin in the fungal cell walls, as a bright cerulean color, and lactophenol acts as a mountant.
Lactophenol Cotton Blue (LPCB) Staining method works on the principle of aiding the identification of the fungal cell walls. Fungi are eukaryotic organisms with both macroscopic and microscopic characteristics. The lactophenol cotton blue solution acts as a mounting solution as well as a staining agent.
Fungal cells have both macroscopic as well as microscopic structure. Lactophenol Cotton Blue reagent is used for staining as well as for wet mounting of fungi. The lactophenol cotton blue (LPCB) wet mount preparation is the most widely used method of staining and observing fungi and is simple to prepare.
LACTOPHENOL COTTON BLUE FOR MICROSCOPY For the staining and microscopic identification of fungi. Ingredients. Cotton Blue (Aniline Blue) 0.05 g; Phenol Crystals (C6H5O4) 20 g; Glycerol 40 mL.
