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PEGs are soluble in water and most organic solvents. The reason for for using PEGs with large molecular weight is that smaller PEGs can diffuse through the cell wall and are even taken up by plant cells and distributed through the plant, which may influence the results. larger than 6,000 (PEG 6000) are inert, non-ionic and cell impermeable. to simulate osmotic stress.
The addition of PEG to a protein solution increases the chemical potential of the protein. Precipitation of the protein occurs when its chemical potential exceeds the level of a saturated solution.
Polyethylene glycols (PEGs) are condensation polymers of ethylene oxide and water with the general formula H(OCH2CH2)nOH. They are the most commercially important type of polyether. The low molecular weight compounds up to 700 are colorless, odorless viscous liquids with a freezing point from -10˚C (diethylene gycol), while polymerized compounds with higher molecular weight than 1,000 are wax like solids with melting point up to 67˚C. While PEGs with different molecular weights find use in different applications and have different physical properties (e.g. viscosity) due to chain length effects, their chemical properties are nearly identical.
The numbers that are often included in the names of PEGs indicate their average molecular weights, e.g. a PEG with n=9 would have an average molecular weight of approximately 400 and would be labeled PEG 400. Most PEGs include molecules with a distribution of molecular weights, i.e. they are polydisperse.
Color of Solution, 5g/50ml of Water SlightlyHazy
Viscosity @98.9˚C250-390 Cst
pH, 5% Solution in Water4.5-7.5
Residue on ignition Max 0.1 %wt
Assay (Average Molecular Weight) 5400-6600 g/mol
Heavy metals Max5 ppm
Ethylene Oxide & 1,4-Dioxane Max.10
Polyethylene glycols are non-toxic, odorless, neutral, lubricating, nonvolatile and nonirritating and are used in a variety of pharmaceuticals and in medications as a solvent, dispersing agent, ointment and suppository bases, vehicle, and tablet excipient.
Melting Point:
PEG 300 = -11°C
PEG 400 = 4-8 °C
PEG 600 = 20-25 °C
PEG 1500 = 44-48 °C
PEG 4000 = 54-58 °C
PEG 6000 = 56-63°C
Polyethylene glycol, referred to as PEG, is used as an inactive ingredient in the pharmaceutical industry as a solvent, plasticizer, surfactant, ointments and suppository base, and tablet and capsule lubricant. ... PEG is also available as a bowel prep for colonoscopy procedures and as a laxative
The reason for for using PEGs with large molecular weight is that smaller PEGs can diffuse through the cell wall and are even taken up by plant cells and distributed through the plant, which may influence the results. larger than 6,000 (PEG 6000) are inert, non-ionic and cell impermeable. to simulate osmotic stress.
